MaxPro

Description

Effect of protease on body weight gain (BWG), feed intake (FI), feed conversion ratio (FCR), and mortality of broilers

Parameters	Age (days)	Treatments*						p-value
		PC	NC	NC (on top 50ml/ton)	NC (on top 100ml/ton)	Matrix (50ml/ ton)	Matrix (100ml/ ton)
		1	2	3	4	5	6
BWG (g/bird)	01-10	280ab	282ab	287ab	293a	289a	273b	0.008
	11-21	669	682	666	693	683	678	0.381
	22-28	610	589	578	595	597	604	0.675
	29-35	689	704	710	704	690	677	0.936
	01-35	2252	2277	2256	2299	2262	2243	0.797
FI (g/bird)	01-10	338	337	350	345	343	337	0.341
	11-21	965	1004	983	1007	1021	994	0.024
	22-28	967	949	946	973	973	980	0.683
	29-35	1189	1174	1190	1188	1198	1178	0.975
	01-35	3463	3492	3500	3539	3537	3508	0.768
FCR	01-10	1.21ab	1.19ab	1.22ab	1.18b	1.19b	1.24a	0.008
	11-21	1.46	1.48	1.49	1.46	1.50	1.47	0.090
	22-28	1.59	1.63	1.66	1.64	1.63	1.63	0.334
	29-35	1.73	1.77	1.69	1.72	1.75	1.78	0.734
	01-35	1.54	1.55	1.58	1.55	1.57	1.57	0.489
Mortality (%)	01-10	0.69	0.00	0.00	0.00	0.00	0.69	0.584
	11-21	1.39	0.69	1.39	1,39	0.69	0.00	0.709
	22-28	0.00	0.69	0.69	0.00	0.00	0.00	0.558
	29-35	0.00	0.72	0.87	0.72	0.00	0.69	0.825
	01-35	2.08	2.08	3.47	2.78	0.69	1.39	0.662
EPEF **	At 35	409	411	395	412	410	402	0.806

^*PC= positive control diet with protease enzyme (as a recommended dose by manufacturer); NC = negative control diet without protease enzyme.

^**European production efficiency factor = liveability (%)x live weight (kg)/age at depletion (d) xFCRx100.

^a-bMeans with different letters in rows differ significantly (P<0.05).

 

Nutrient digestibility

The results of the evaluation of the effect of protease supplementation on digestibility indices are shown in Table 4.3. Crude protein and crude fat digestibility were found to be significantly affected by dietary interventions (P=0.032 and P=0.041, respectively). However, neither the digestibility of dry matter (P=0.472) nor that of ash (P=0.167) showed appreciable changes.

The significant improvement in fat digestibility may be attributed to the byproduct of protein breakdown. The protease supplementation may have facilitated the breakdown of large protein molecules within the chyme complex, increasing the total surface area of lipid molecules available for the production of micelles.

These results show that protease supplementation can improve the digestibility of certain nutrients, particularly crude protein, and fat. The observed rises in digestibility indices point to a potential role for protease enzymes in enhancing nutrient utilization and absorption in the gastrointestinal tract. However, more investigation is required to clarify the underlying mechanisms and comprehend the effects of protease supplementation on nutrient absorption.

Effect of protease on nutrients digestibility (%) at 35 days

Parameters	Treatments*						p-value
	PC	NC	NC (on top 50ml/ton)	NC (on top 100ml/ton)	Matrix (50ml/ton)	Matrix (100ml/ton)
	1	2	3	4	5	6
Dry matter	91.06	91.37	91.47	91.19	91.86	91.62	0.472
Crude protein	69.25a	65.37b	69.48a	71.01a	70.77a	69.53a	0.032
Crude fat	91.23a	88.5b	91.10a	91.15a	91.30 a	91.02a	0.041
Total ash	38.61	38.24	38.75	38.38	38.74	38.53	0.167

^*PC= positive control diet with protease enzyme (as recommended dose by manufacturer); NC = negative control diet without protease enzyme

^a-bMeans with different letters in rows differ significantly (P<0.05).

The current study’s results align with those published by Fru-Nji et al. (2011).  In their research, broilers in the positive control group received the same diet as the control group, with 200 mg/kg of protease added. During the starter phase, the addition of enzymes significantly affected both body weight gain (BWG) and feed conversion ratio (FCR) (P=0.002). However, during the grower phase, the effects of enzyme supplementation on growth parameters were not statistically significant.

Conclusion

• It is concluded that protease can be on toped in diets up to 100 g/ton of feed which improved bird performance in most of the parameters without any harmful effect on bird health.
• Protease enzyme supplementation doesn’t affect antibody titers of N.D, I.B and Avian Influenza at all so further studies need to be conducted for the endorsement of this finding.
• Microbial colonies remain un affected in Caecum where clostridia was found to be completely negative and at the same time Salmonella and E.coli were not influenced by the addition of protease enzyme.
• Since all the carcass characteristics remained to be insignificant in all treatments so this needs to be further studied and analyzed deeply to understand the effect of protease inclusion on any of these characteristics in particular.
• Protease supplementation not only alters Crude Protein digestibility but at the same time Crude Fat digestion also improved significantly.
• Overall health status of the bird is not effected by the addition of exogenous protease as mortality was found to be in normal range and same is the case with intestinal microflora and antibody titers.

Recommendations

• Protease should be supplemented @100ml/ton and especially in starter diet.
• Protease not just only improves protein digestion but it also helps in fat digestion as well.
• Protease Supplementation with matrix values is of no significance as such.
• It has no significant effect on carcass characteristics as such. (More important for chicken processing units).
• No effect on Antibody Titers (ND,AI, IB)
• Since, Clostridia came to be negative in all the groups and this negates the claim that undigested protein in distal part of GIT proliferate pathogenic bacteria there.